Journal: Cancer Gene Therapy

Article Title: Lycium barbarum glycopeptide targets PER2 to inhibit lipogenesis in glioblastoma by downregulating SREBP1c

doi: 10.1038/s41417-023-00611-4

Figure Lengend Snippet: A RT-qPCR was performed to detected 7 clock genes in U87 cells treated with 200 µg/ml of LbGP for 6 h. B , C Protein expression levels of PER2 in the control and LbGP treated U87 cells for 6 h. D – F U87 cells were treated with LbGP for 6 h, H89 concentration was at 30 µM. The expression of protein was measured by western blotting. The data are represented as the mean ± SD of three replicates. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The cells were transfected with PER2 shRNA lentiviral particles and PER2 overexpression lentiviral particles (Genechem Biotechnology, Shanghai) at a multiplicity of infection (MOI) of 10, replaced with new medium 16 hours after transfection.

Techniques: Quantitative RT-PCR, Expressing, Control, Concentration Assay, Western Blot

Journal: Cancer Gene Therapy

Article Title: Lycium barbarum glycopeptide targets PER2 to inhibit lipogenesis in glioblastoma by downregulating SREBP1c

doi: 10.1038/s41417-023-00611-4

Figure Lengend Snippet: A, B The expression of PER2 was measured by western blotting after silenced by RNA lentivirus. C shNC or shPer2 U87 Cells were treated with LbGP or PBS for 24 h, and the cell viability was determined by CCK-8 assay. D , E Migration assays of shNC or shPer2 U87 cells treated with 200 µg/ml of LbGP for 24 h. F, G Transwell invision assays of shNC or shPer2 U87 cells treated with 200 µg/ml of LbGP for 24 h. H Schedule of inject cells、feed LbGP and IVIS. I – K IVIS image and total flux results on day 7. L – N IVIS image and total flux results on day 28 of different groups. O, P Survival rates and time of different groups. The data are represented as the mean ± SD of three replicates. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The cells were transfected with PER2 shRNA lentiviral particles and PER2 overexpression lentiviral particles (Genechem Biotechnology, Shanghai) at a multiplicity of infection (MOI) of 10, replaced with new medium 16 hours after transfection.

Techniques: Expressing, Western Blot, CCK-8 Assay, Migration

Journal: Cancer Gene Therapy

Article Title: Lycium barbarum glycopeptide targets PER2 to inhibit lipogenesis in glioblastoma by downregulating SREBP1c

doi: 10.1038/s41417-023-00611-4

Figure Lengend Snippet: A Process of lipid synthesis and metabolism. B RT‒qPCR was performed to detect 4 lipid-related genes in the U87 cells treated with 200 µg/ml LbGP for 6 h. C, D Western bolt of the U87 cells treated with 200 µg/ml LbGP for 6 h. E ICF of the U87 cells treated with 200 µg/ml LbGP for 6 h. F – I Western blot was performed to assess protein expression of the shNC or shPer2 U87 cells treated with 200 µg/ml LbGP or PBS for 6 h. J The expression levels of SREBP1c were examined by immunohistochemistry in animal specimens. K – N The protein expression of SREBP1c, FASN and PER2 was measured by western blotting. The data are represented as the mean ± SD of three replicates. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The cells were transfected with PER2 shRNA lentiviral particles and PER2 overexpression lentiviral particles (Genechem Biotechnology, Shanghai) at a multiplicity of infection (MOI) of 10, replaced with new medium 16 hours after transfection.

Techniques: Western Blot, Expressing, Immunohistochemistry

Journal: Cancer Gene Therapy

Article Title: Lycium barbarum glycopeptide targets PER2 to inhibit lipogenesis in glioblastoma by downregulating SREBP1c

doi: 10.1038/s41417-023-00611-4

Figure Lengend Snippet: A The expression levels of PER2 and SREBP1c were examined by immunohistochemistry in glioma tissue of different grades. B, C PER2 and SREBP1c immunohistochemistry score of high or low in glioma tissue from 48 patients. The correlation was assessed with χ2 test. ** P < 0.01. D, E Patients’ Survival rates with high expression of PER2 or SREBP1c. F – L Protein expression of FASN、SREBP1c and PER2 in primary glioma cells from 6 patients.

Article Snippet: The cells were transfected with PER2 shRNA lentiviral particles and PER2 overexpression lentiviral particles (Genechem Biotechnology, Shanghai) at a multiplicity of infection (MOI) of 10, replaced with new medium 16 hours after transfection.

Techniques: Expressing, Immunohistochemistry

Journal: Cancer Gene Therapy

Article Title: Lycium barbarum glycopeptide targets PER2 to inhibit lipogenesis in glioblastoma by downregulating SREBP1c

doi: 10.1038/s41417-023-00611-4

Figure Lengend Snippet: A – F Overexpression of Per2 inhibited the expression of SREBP1c via downregulation of the PI3K/AKT/mTOR signaling pathway. Expression levels of PER2, AKT, p-AKT, mTOR, p-mTOR, SREBP1c, was detected with Western blot. The data are represented as the mean ± SD of three replicates. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The cells were transfected with PER2 shRNA lentiviral particles and PER2 overexpression lentiviral particles (Genechem Biotechnology, Shanghai) at a multiplicity of infection (MOI) of 10, replaced with new medium 16 hours after transfection.

Techniques: Over Expression, Expressing, Western Blot